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Effects of oxidation in the power of chemically skinned rat soleus fibres

Gilliver, Sally F and Jones, David A and Rittweger, Jörn and Degens, Hans (2010) Effects of oxidation in the power of chemically skinned rat soleus fibres. Journal of Musculoskeletal and Neuronal Interactions, 10 (4), pp. 245-248.

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Oxidation alters calcium sensitivity, and decreases maximum isometric force (Po) and shortening velocity (Vmax) of single muscle fibres. To examine the effect of oxidation on the curvature of the force-velocity relationship, which determines muscle power in addition to Po and Vmax, skinned rat type I fibres were maximally activated at 15ºC in a solution with pCa 4.5 and subjected to isotonic contractions before and after 4-min incubation in 50 mM H2O2 (n=10) or normal relaxing solution (n=3). In five oxidised and four control fibres the rate of force redevelopment (ktr), following a rapid release and re-stretch, was measured. This gives a measure of the sum of the rate constants for cross-bridge attachment (f) and detachment (g1): (f+g1). H2O2 reduced Po, Vmax and ktr by 19%, 21% and 24% respectively (P<0.001), while the shape of the force-velocity relationship was unchanged. Fitting data to the Huxley cross-bridge model suggested that oxidation decreased both the rate constant for cross-bridge attachment (f), and detachment of negatively strained cross-bridges (g2), similar to the effect of reduced activation1. This suggests that oxidative modification is a possible cause of the variation in contractile properties between muscle fibres of the same type2.

Document Type:Article
Title:Effects of oxidation in the power of chemically skinned rat soleus fibres
AuthorsInstitution or Email of AuthorsAuthors ORCID
Gilliver, Sally FManchester Metropolitan UniversityUNSPECIFIED
Jones, David AManchester Metropolitan UniversityUNSPECIFIED
Rittweger, Jörnjoern.rittweger@dlr.deUNSPECIFIED
Degens, HansManchester Metropolitan UniversityUNSPECIFIED
Date:December 2010
Journal or Publication Title:Journal of Musculoskeletal and Neuronal Interactions
Refereed publication:Yes
In Open Access:Yes
In ISI Web of Science:Yes
Page Range:pp. 245-248
Keywords:Skeletal Muscle, Skinned Fibres, Power, Force-Velocity Relationship, Oxidation
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Integrative Studien (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Rittweger, Jörn
Deposited On:13 Dec 2010 09:46
Last Modified:04 Apr 2013 16:26

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