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Effects of oxidation in the power of chemically skinned rat soleus fibres

Gilliver, Sally F and Jones, David A and Rittweger, Jörn and Degens, Hans (2010) Effects of oxidation in the power of chemically skinned rat soleus fibres. Journal of Musculoskeletal and Neuronal Interactions, 10 (4), pp. 245-248.

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Abstract

Oxidation alters calcium sensitivity, and decreases maximum isometric force (Po) and shortening velocity (Vmax) of single muscle fibres. To examine the effect of oxidation on the curvature of the force-velocity relationship, which determines muscle power in addition to Po and Vmax, skinned rat type I fibres were maximally activated at 15ºC in a solution with pCa 4.5 and subjected to isotonic contractions before and after 4-min incubation in 50 mM H2O2 (n=10) or normal relaxing solution (n=3). In five oxidised and four control fibres the rate of force redevelopment (ktr), following a rapid release and re-stretch, was measured. This gives a measure of the sum of the rate constants for cross-bridge attachment (f) and detachment (g1): (f+g1). H2O2 reduced Po, Vmax and ktr by 19%, 21% and 24% respectively (P<0.001), while the shape of the force-velocity relationship was unchanged. Fitting data to the Huxley cross-bridge model suggested that oxidation decreased both the rate constant for cross-bridge attachment (f), and detachment of negatively strained cross-bridges (g2), similar to the effect of reduced activation1. This suggests that oxidative modification is a possible cause of the variation in contractile properties between muscle fibres of the same type2.

Document Type:Article
Title:Effects of oxidation in the power of chemically skinned rat soleus fibres
Authors:
AuthorsInstitution or Email of Authors
Gilliver, Sally FManchester Metropolitan University
Jones, David AManchester Metropolitan University
Rittweger, Jörnjoern.rittweger@dlr.de
Degens, HansManchester Metropolitan University
Date:December 2010
Journal or Publication Title:Journal of Musculoskeletal and Neuronal Interactions
Refereed publication:Yes
In Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:10
Page Range:pp. 245-248
Status:Published
Keywords:Skeletal Muscle, Skinned Fibres, Power, Force-Velocity Relationship, Oxidation
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Integrative Studien (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Jörn Rittweger
Deposited On:13 Dec 2010 09:46
Last Modified:04 Apr 2013 16:26

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