DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Contact | Deutsch
Fontsize: [-] Text [+]

Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation

Moriggi, Manuela and Vasso, Michele and Fania, Chiara and Capitanio, Daniele and Bonifacio, Gaetano and Salanova, Michele and Blottner, Dieter and Rittweger, Jörn and Felsenberg, Dieter and Cerretelli, Paolo and Gelfi, Cecilia (2010) Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation. Proteomics, 10, pp. 3756-3774.

WarningThere is a more recent version of this item available.

PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader


The present investigation, the first in the field, was aimed at analyzing differentially, on individual samples, the effects of 55 days of horizontal bed rest, a model for microgravity, on myosin heavy and myosin light chain isoforms distribution (by SDS) and on the proteome (by 2-D DIGE and MS) in the vastus lateralis (VL), a mixed type II/I (50:50%) head of the quadriceps and in the calf soleus (SOL), a predominantly slow (35:65%) twitch muscle. Two separate studies were performed on six subjects without (BR) and six with resistive vibration exercise (RVE) countermeasures, respectively. Both VL and SOL underwent in BR decrements of 15% in cross-sectional area and of 22% in maximal torque that were prevented by RVE. Myosin heavy chain distribution showed increased type I and decreased type IIA in BR both in VL and in SOL, the opposite with RVE. A substantial downregulation of proteins involved in aerobic metabolism characterized both in SOL and VL in BR. RVE reversed the pattern more in VL than in SOL, whereas proteins involved in anaerobic glycolysis were upregulated. Proteins from the Z-disk region and from costamers were differently dysregulated during bed rest (both BR and RVE), particularly in VL.

Document Type:Article
Title:Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation
AuthorsInstitution or Email of Authors
Moriggi, ManuelaUniversity of Milan
Vasso, MicheleUniversity of Milan
Fania, ChiaraUniversity of Milan
Capitanio, DanieleUniversity of Milan
Bonifacio, GaetanoUniversity of Milan
Salanova, MicheleCharité - University Medicine Berlin
Blottner, DieterCharité - University Medicine Berlin
Rittweger, Jörnjoern.rittweger@dlr.de
Felsenberg, DieterCharité - University Medicine Berlin
Cerretelli, PaoloCNR Milan
Gelfi, CeciliaUniversity of Milan
Journal or Publication Title:Proteomics
Refereed publication:Yes
In ISI Web of Science:Yes
Page Range:pp. 3756-3774
Keywords:2-D DIGE / Bed rest / Biomedicine / Exercise countermeasures / Mass Spectrometry / Muscle
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Integrative Studien (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Jörn Rittweger
Deposited On:23 Nov 2010 13:10
Last Modified:12 Dec 2013 21:01

Available Versions of this Item

Repository Staff Only: item control page

Help & Contact
electronic library is running on EPrints 3.3.12
Copyright © 2008-2012 German Aerospace Center (DLR). All rights reserved.