Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation

Moriggi, Manuela and Vasso, Michele and Fania, Chiara and Capitanio, Daniele and Bonifacio, Gaetano and Salanova, Michele and Blottner, Dieter and Rittweger, Jörn and Felsenberg, Dieter and Cerretelli, Paolo and Gelfi, Cecilia (2010) Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation. Proteomics, 10, pp. 3756-3774.

There is a more recent version of this item available.

Preview

PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
424Kb

Abstract

The present investigation, the first in the field, was aimed at analyzing differentially, on individual samples, the effects of 55 days of horizontal bed rest, a model for microgravity, on myosin heavy and myosin light chain isoforms distribution (by SDS) and on the proteome (by 2-D DIGE and MS) in the vastus lateralis (VL), a mixed type II/I (50:50%) head of the quadriceps and in the calf soleus (SOL), a predominantly slow (35:65%) twitch muscle. Two separate studies were performed on six subjects without (BR) and six with resistive vibration exercise (RVE) countermeasures, respectively. Both VL and SOL underwent in BR decrements of 15% in cross-sectional area and of 22% in maximal torque that were prevented by RVE. Myosin heavy chain distribution showed increased type I and decreased type IIA in BR both in VL and in SOL, the opposite with RVE. A substantial downregulation of proteins involved in aerobic metabolism characterized both in SOL and VL in BR. RVE reversed the pattern more in VL than in SOL, whereas proteins involved in anaerobic glycolysis were upregulated. Proteins from the Z-disk region and from costamers were differently dysregulated during bed rest (both BR and RVE), particularly in VL.

Document Type:

Article

Title:

Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation

Authors:

Authors	Institution or Email of Authors
Moriggi, Manuela	University of Milan
Vasso, Michele	University of Milan
Fania, Chiara	University of Milan
Capitanio, Daniele	University of Milan
Bonifacio, Gaetano	University of Milan
Salanova, Michele	Charité - University Medicine Berlin
Blottner, Dieter	Charité - University Medicine Berlin
Rittweger, Jörn	joern.rittweger@dlr.de
Felsenberg, Dieter	Charité - University Medicine Berlin
Cerretelli, Paolo	CNR Milan
Gelfi, Cecilia	University of Milan

Date:

2010

Journal or Publication Title:

Proteomics

Refereed publication:

Yes

In SCOPUS:

Yes

In ISI Web of Science:

Yes

Volume:

Page Range:

pp. 3756-3774

Status:

Published

Keywords:

2-D DIGE / Bed rest / Biomedicine / Exercise countermeasures / Mass Spectrometry / Muscle

HGF - Research field:

Aeronautics, Space and Transport

HGF - Program:

Space

HGF - Program Themes:

W FR - Forschung unter Weltraumbedingungen

DLR - Research area:

Space

DLR - Program:

W FR - Forschung unter Weltraumbedingungen

DLR - Research theme (Project):

W - Vorhaben Integrative Studien (old)

Location:

Köln-Porz

Institutes and Institutions:

Institute of Aerospace Medicine > Space Physiology

Deposited By:

Jörn Rittweger

Deposited On:

23 Nov 2010 13:10

Last Modified:

26 Feb 2013 15:05

Available Versions of this Item

Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation. (deposited 23 Nov 2010 13:10) [Currently Displayed]
- Reporting whole-body vibration intervention studies: Recommendations of the International Society of Musculoskeletal and Neuronal Interactions. (deposited 11 Oct 2010 09:07)
  - The 2nd Berlin BedRest study: protocol and implementation. (deposited 11 Oct 2010 09:25)

Repository Staff Only: item control page