elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Contact | Deutsch
Fontsize: [-] Text [+]

Validation of a Nylon-Flocked-Swab Protocol for Efficient Recovery of Bacterial Spores from Smooth and Rough Surfaces

Probst, Alexander and Facius, Rainer and Wirth, Reinhard and Moissl-Eichinger, Christine (2010) Validation of a Nylon-Flocked-Swab Protocol for Efficient Recovery of Bacterial Spores from Smooth and Rough Surfaces. Applied and Environmental Microbiology, 76 (15), pp. 5148-5158. American Society for Microbiology. DOI: 10.1128/AEM.00399-10.

Full text not available from this repository.

Abstract

In order to meet planetary-protection requirements, culturable bacterial spore loads are measured representatively for the total microbial contamination of spacecraft. However, the National Aeronautics and Space Administration’s (NASA’s) cotton swab protocols for spore load determination have not changed for decades. To determine whether a more efficient alternative was available, a novel swab was evaluated for recovery of different Bacillus atrophaeus spore concentrations on stainless steel and other surfaces. Two protocols for the nylon-flocked swab (NFS) were validated and compared to the present NASA standard protocol. The results indicate that the novel swab protocols recover 3- to 4-fold more (45.4% and 49.0% recovery efficiency) B. atrophaeus spores than the NASA standard method (13.2%). Moreover, the nylon-flocked-swab protocols were superior in recovery efficiency for spores of seven different Bacillus species, including Bacillus anthracis Sterne (recovery efficiency, 20%). The recovery efficiencies for B. atrophaeus spores from different surfaces showed a variation from 5.9 to 62.0%, depending on the roughness of the surface analyzed. Direct inoculation of the swab resulted in a recovery rate of about 80%, consistent with the results of scanning electron micrographs that allowed detailed comparisons of the two swab types. The results of this investigation will significantly contribute to the cleanliness control of future life detection missions and will provide significant improvement in detection of B. anthracis contamination for law enforcement and security efforts.

Document Type:Article
Title:Validation of a Nylon-Flocked-Swab Protocol for Efficient Recovery of Bacterial Spores from Smooth and Rough Surfaces
Authors:
AuthorsInstitution or Email of Authors
Probst, Alexander Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
Facius, Rainer UNSPECIFIED
Wirth, Reinhard Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
Moissl-Eichinger, Christine Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
Date:2010
Journal or Publication Title:Applied and Environmental Microbiology
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:76
DOI:10.1128/AEM.00399-10
Page Range:pp. 5148-5158
Publisher:American Society for Microbiology
Status:Published
Keywords:Planetary Protection, Bacterial Spores, Nylon-Flocked-Swab Protocol
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:16 Sep 2010 14:47
Last Modified:04 Apr 2013 16:22

Repository Staff Only: item control page

Browse
Search
Help & Contact
Informationen
electronic library is running on EPrints 3.3.12
Copyright © 2008-2012 German Aerospace Center (DLR). All rights reserved.