elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Impressum | Kontakt | English
Schriftgröße: [-] Text [+]

Validation of a Nylon-Flocked-Swab Protocol for Efficient Recovery of Bacterial Spores from Smooth and Rough Surfaces

Probst, Alexander und Facius, Rainer und Wirth, Reinhard und Moissl-Eichinger, Christine (2010) Validation of a Nylon-Flocked-Swab Protocol for Efficient Recovery of Bacterial Spores from Smooth and Rough Surfaces. Applied and Environmental Microbiology, 76 (15), Seiten 5148-5158. American Society for Microbiology. DOI: 10.1128/AEM.00399-10.

Dieses Archiv kann nicht den gesamten Text zur Verfügung stellen.

Kurzfassung

In order to meet planetary-protection requirements, culturable bacterial spore loads are measured representatively for the total microbial contamination of spacecraft. However, the National Aeronautics and Space Administration’s (NASA’s) cotton swab protocols for spore load determination have not changed for decades. To determine whether a more efficient alternative was available, a novel swab was evaluated for recovery of different Bacillus atrophaeus spore concentrations on stainless steel and other surfaces. Two protocols for the nylon-flocked swab (NFS) were validated and compared to the present NASA standard protocol. The results indicate that the novel swab protocols recover 3- to 4-fold more (45.4% and 49.0% recovery efficiency) B. atrophaeus spores than the NASA standard method (13.2%). Moreover, the nylon-flocked-swab protocols were superior in recovery efficiency for spores of seven different Bacillus species, including Bacillus anthracis Sterne (recovery efficiency, 20%). The recovery efficiencies for B. atrophaeus spores from different surfaces showed a variation from 5.9 to 62.0%, depending on the roughness of the surface analyzed. Direct inoculation of the swab resulted in a recovery rate of about 80%, consistent with the results of scanning electron micrographs that allowed detailed comparisons of the two swab types. The results of this investigation will significantly contribute to the cleanliness control of future life detection missions and will provide significant improvement in detection of B. anthracis contamination for law enforcement and security efforts.

Dokumentart:Zeitschriftenbeitrag
Titel:Validation of a Nylon-Flocked-Swab Protocol for Efficient Recovery of Bacterial Spores from Smooth and Rough Surfaces
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Probst, Alexander Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
Facius, Rainer NICHT SPEZIFIZIERT
Wirth, Reinhard Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
Moissl-Eichinger, Christine Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
Datum:2010
Erschienen in:Applied and Environmental Microbiology
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:76
DOI :10.1128/AEM.00399-10
Seitenbereich:Seiten 5148-5158
Verlag:American Society for Microbiology
Status:veröffentlicht
Stichwörter:Planetary Protection, Bacterial Spores, Nylon-Flocked-Swab Protocol
HGF - Forschungsbereich:Verkehr und Weltraum (alt)
HGF - Programm:Weltraum (alt)
HGF - Programmthema:W FR - Forschung unter Weltraumbedingungen (alt)
DLR - Schwerpunkt:Weltraum
DLR - Forschungsgebiet:W FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):W - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:16 Sep 2010 14:47
Letzte Änderung:04 Apr 2013 16:22

Nur für Mitarbeiter des Archivs: Kontrollseite des Eintrags

Blättern
Suchen
Hilfe & Kontakt
Informationen
electronic library verwendet EPrints 3.3.12
Copyright © 2008-2013 Deutsches Zentrum für Luft- und Raumfahrt (DLR). Alle Rechte vorbehalten.