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UV photoreactions of the extremely haloalkaliphilic euryarchaeon Natronomonas pharaonis

Moeller, Ralf and Reitz , Guenther and Douki, Thierry and Cadet, Jean and Horneck, Gerda and Stan-Lotter, Helga (2010) UV photoreactions of the extremely haloalkaliphilic euryarchaeon Natronomonas pharaonis. Fems Microbiology Ecology, 73 (2), pp. 271-277. Federation of European Microbiological Societies, Published by Blackwell Publishing Ltd.. DOI: 10.1111/j.1574-6941.2010.00893.x.

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Abstract

The objective of this study was to investigate the photobiological responses of the haloalkaliphilic euryarchaeon Natronomonas pharaonis to environmentally relevant polychromatic UV radiation, simulating either the present UV radiation climate (λ>290 nm) or that of the early Earth (λ>220 nm), and to monochromatic UVC radiation (λ=254 nm) for comparison with the literature data. UV-induced bipyrimidine DNA photoproducts were determined using a sensitive and accurate HPLC tandem mass spectrometry assay, allowing to identify and quantify each type of photoproducts formed in the DNA of a UV-irradiated halophilic archaeon. The thymine cytosine (TC) pyrimidine (6-4) pyrimidone photoproduct and the TC cyclobutane pyrimidine dimer accounted for almost 80% of the total induced DNA photolesions, regardless of the wavelength range tested. These prominent formation rates of TC photoproducts correlated with the genomic frequencies of TC dinucleotides in N. pharaonis.

Document Type:Article
Title:UV photoreactions of the extremely haloalkaliphilic euryarchaeon Natronomonas pharaonis
Authors:
AuthorsInstitution or Email of Authors
Moeller, Ralf UNSPECIFIED
Reitz , Guenther UNSPECIFIED
Douki, Thierry Commissariat á l’Énergie Atomique (CEA), Institut Nanosciences et Cryogénie, SCIB-UMR-E n°3 (CEA/UJF), Laboratoire ‘Lésions des Acides Nucléiques’, Grenoble, France
Cadet, Jean Commissariat á l’Énergie Atomique (CEA), Institut Nanosciences et Cryogénie, SCIB-UMR-E n°3 (CEA/UJF), Laboratoire ‘Lésions des Acides Nucléiques’, Grenoble, France
Horneck, Gerda UNSPECIFIED
Stan-Lotter, Helga Department of Microbiology, Division of Molecular Biology, University of Salzburg, Salzburg, Austria
Date:2010
Journal or Publication Title:Fems Microbiology Ecology
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:73
DOI:10.1111/j.1574-6941.2010.00893.x
Page Range:pp. 271-277
Publisher:Federation of European Microbiological Societies, Published by Blackwell Publishing Ltd.
Status:Published
Keywords:halophilic archaea, Natronomonas pharaonis, environmental UV radiation, germicidal UV radiation, DNA photoproducts, photoreaction
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:01 Sep 2010 11:23
Last Modified:04 Apr 2013 16:22

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