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Astrobiological Aspects of the Mutagenesis of Cosmic Radiation on Bacterial Spores

Moeller, Ralf und Reitz, Guenther und Berger, Thomas und Okayasu, Ryuichi und Nicholson, Wayne L. und Horneck, Gerda (2010) Astrobiological Aspects of the Mutagenesis of Cosmic Radiation on Bacterial Spores. Astrobiology, 10 (5), Seiten 509-521. Mary Ann Liebert, Inc.. DOI: 10.1089/ast.2009.0429.

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Kurzfassung

Based on their unique resistance to various space parameters, Bacillus endospores are one of the model systems used for astrobiological studies. In this study, spores of B. subtilis were used to study the effects of galactic cosmic radiation (GCR) on spore survival and induced mutagenesis. In interplanetary space, outside Earth's protective magnetic field, spore-containing rocks would be exposed to bombardment by high-energy charged particle radiation from galactic sources and from the Sun, which consists of photons (X-rays, γ rays), protons, electrons, and heavy, high-energy charged (HZE) particles. B. subtilis spores were irradiated with X-rays and accelerated heavy ions (helium, carbon, silicon and iron) in the linear energy transfer (LET) range of 2–200keV/μm. Spore survival and the rate of the induced mutations to Rifampicin-resistance depended on the LET of the applied species of ions and radiation, whereas the exposure to high-energy charged particles, for example, iron ions, led to a low level of spore survival and increased frequency of mutation to Rifampicin-resistance compared to low-energy charged particles and X-rays. Twenty-one Rifampicin-resistance mutant spores were isolated from X-ray and heavy ion–irradiated samples. Nucleotide sequencing located the Rifampicin-resistance mutations in the rpoB gene encoding the β-subunit of RNA polymerase. Most mutations were primarily found in Cluster I and were predicted to result in amino acid changes at residues Q469L, A478V, and H482P/Y. Four previously undescribed alleles in B. subtilis rpoB were isolated: L467P, R484P, and A488P in Cluster I and H507R in the spacer between Clusters I and II. The spectrum of Rifampicin-resistance mutations arising from spores exposed to components of GCR is distinctly different from those of spores exposed to simulated space vacuum and martian conditions.

Dokumentart:Zeitschriftenbeitrag
Titel:Astrobiological Aspects of the Mutagenesis of Cosmic Radiation on Bacterial Spores
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Moeller, Ralf NICHT SPEZIFIZIERT
Reitz, Guenther NICHT SPEZIFIZIERT
Berger, Thomas NICHT SPEZIFIZIERT
Okayasu, Ryuichi National Institute of Radiological Sciences (NIRS), Research Center for Charged Particle Therapy, Heavy-Ion Radiobiology Research Group, Chiba-shi, Japan.
Nicholson, Wayne L. University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA.
Horneck, Gerda NICHT SPEZIFIZIERT
Datum:2010
Erschienen in:Astrobiology
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:10
DOI :10.1089/ast.2009.0429
Seitenbereich:Seiten 509-521
Verlag:Mary Ann Liebert, Inc.
Status:veröffentlicht
Stichwörter:Bacillus, Spores, High-energy charged particle bombardment, Lithopanspermia
HGF - Forschungsbereich:Verkehr und Weltraum (alt)
HGF - Programm:Weltraum (alt)
HGF - Programmthema:W FR - Forschung unter Weltraumbedingungen (alt)
DLR - Schwerpunkt:Weltraum
DLR - Forschungsgebiet:W FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):W - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:16 Jul 2010 09:21
Letzte Änderung:20 Mär 2013 19:42

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