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Genomic bipyrimidine nucleotide frequency and microbial reactions to germicidal UV radiation

Moeller, Ralf and Douki, Thierry and Rettberg, Petra and Reitz, Guenther and Cadet, Jean and Nicholson, Wayne L. and Horneck, Gerda (2010) Genomic bipyrimidine nucleotide frequency and microbial reactions to germicidal UV radiation. Archives of Microbiology, 192 (7), pp. 521-529. Springer-Verlag. DOI: 10.1007/s00203-010-0579-3. ISSN 0302-8933.

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Abstract

The role of the genomic bipyrimidine nucleotide frequency in pyrimidine dimer formation caused by germicidal UV radiation was studied in three microbial reference organisms (Escherichia coli K12, Deinococcus radiodurans R1, spores and cells of Bacillus subtilis 168). The sensitive HPLC tandem mass spectrometry assay was used to identify and quantify the different bipyrimidine photoproducts induced in the DNA of microorganisms by germicidal UV radiation. The yields of photoproducts per applied fluence were very similar among vegetative cells but twofold reduced in spores. This similarity in DNA photoreactivity greatly contrasted with the 11-fold range determined in the fluence causing a decimal reduction of survival. It was also found that the spectrum of UV-induced bipyrimidine lesions was species-specific and the formation rates of bi-thymine and bi-cytosine photoproducts correlated with the genomic frequencies of thymine and cytosine dinucleotides in the bacterial model systems.

Document Type:Article
Title:Genomic bipyrimidine nucleotide frequency and microbial reactions to germicidal UV radiation
Authors:
AuthorsInstitution or Email of Authors
Moeller, Ralf UNSPECIFIED
Douki, Thierry Commissariat à l'Energie Atomique (CEA), Institut Nanosciences et Cryogénie, SCIB-UMR-E n°3 (CEA/UJF), Laboratoire ‘‘Lésions des Acides Nucléiques’’, Grenoble, France
Rettberg, Petra UNSPECIFIED
Reitz, Guenther UNSPECIFIED
Cadet, Jean Commissariat à l'Energie Atomique (CEA), Institut Nanosciences et Cryogénie, SCIB-UMR-E n°3 (CEA/UJF), Laboratoire ‘‘Lésions des Acides Nucléiques’’, Grenoble, France
Nicholson, Wayne L. Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center 32899, FL, USA
Horneck, Gerda UNSPECIFIED
Date:2010
Journal or Publication Title:Archives of Microbiology
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:192
DOI:10.1007/s00203-010-0579-3
Page Range:pp. 521-529
Publisher:Springer-Verlag
ISSN:0302-8933
Status:Published
Keywords:Bacillus spores, Deinococcus radiodurans, Escherichia coli, Germicidal UV radiation, DNA photolesions, Bipyrimidine photoproducts
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:16 Jul 2010 09:28
Last Modified:07 Feb 2013 19:39

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