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Cytotoxicity and Genotoxicity Reporter Systems Based on the Use of Mammalian Cells

Baumstark-Khan, Christa and Hellweg, Christine E. and Reitz, Guenther (2010) Cytotoxicity and Genotoxicity Reporter Systems Based on the Use of Mammalian Cells. Advances in Biochemical Engineering/Biotechnology, 118, pp. 113-151. Springer‐Verlag Berlin Heidelberg. DOI: 10.1007/10_2009_20.

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With the dramatic increase in the number of new agents arising from the chemical, pharmaceutical, and agricultural industries, there is an urgent need to develop assays for rapid evaluation of potential risks to man and environment. The panel of conventional tests used for cytotoxicity and genotoxicity and the strategies to progress from small scale assays to high content screening in toxicology are discussed. The properties of components necessary as sensors and reporters for new reporter assays, and the application of genetic strategies to design assays are reviewed. The concept of cellular reporters is based on the use of promoters of chemical stress-regulated genes ligated to a suitable luminescent or fluorescent reporter gene. Current reporter assays designed from constructs transferred into suitable cell lines are presented.

Document Type:Article
Title:Cytotoxicity and Genotoxicity Reporter Systems Based on the Use of Mammalian Cells
AuthorsInstitution or Email of Authors
Baumstark-Khan, Christa UNSPECIFIED
Hellweg, Christine E. UNSPECIFIED
Reitz, GuentherUNSPECIFIED
Journal or Publication Title:Advances in Biochemical Engineering/Biotechnology
Refereed publication:Yes
In ISI Web of Science:Yes
Page Range:pp. 113-151
Publisher:Springer‐Verlag Berlin Heidelberg
Keywords:Cytotoxicity, DNA damage and repair, Genotoxicity, Mammalian cells, Reporter assays
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:16 Sep 2010 14:51
Last Modified:07 Feb 2013 20:07

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