Linear energy transfer dependent expression of putative radiation responsive genes in human cells
Ruland, Rebecca (2010) Linear energy transfer dependent expression of putative radiation responsive genes in human cells. Master's, FH Bonn-Rhein-Sieg, University of Applied Sciences.
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Manned space travel is one of the main challenges of modern mankind, although the risk of exposure to radiation in space still remains a limiting factor. To estimate the risk of astronauts to develop cancer, molecular mechanisms of the cellular radiation response have to be analyzed. This thesis deals especially with the correlation between quality of radiation in terms of linear energy transfer (LET) and the consequential expression of radiation responsive genes in human embryonic kidney cells (HEK). Besides, factors like cellular survival, cell cycle progression and activation of the transcription factor Nuclear Factor κB (NF-κB) after exposure to radiation with LET ranging from 3 keV/μm (X-rays) to 9674 keV/μm (accelerated heavy ions, in this case Pb-208 ions) were investigated. Survival declined and NF-κB activation increased intensively with rising radiation dose, while a high LET always correlated with a strong response. A significant cell cycle arrest in the G 2 phase indicated active DNA repair, which was observed especially in medium LET range. In contrast, expression levels of radiation responsive genes (GADD45α, GADD45β, NFKBIA, p21) were proven to be higher after high LET irradiation. Some genes (HSF1, UNG, XRCC5) even responded only to high LET radiation. This indicates a dependency of the quality and intensity of cellular radiation response on LET.
|Document Type:||Thesis (Master's)|
|Title:||Linear energy transfer dependent expression of putative radiation responsive genes in human cells|
|Number of Pages:||95|
|Keywords:||LET, Human cells, Space radiation, Cellular radiation response|
|Institution:||FH Bonn-Rhein-Sieg, University of Applied Sciences|
|Department:||Department of Natural Sciences|
|HGF - Research field:||Aeronautics, Space and Transport|
|HGF - Program:||Space|
|HGF - Program Themes:||W FR - Forschung unter Weltraumbedingungen|
|DLR - Research area:||Space|
|DLR - Program:||W FR - Forschung unter Weltraumbedingungen|
|DLR - Research theme (Project):||W - Vorhaben Strahlenbiologie (old)|
|Institutes and Institutions:||Institute of Aerospace Medicine > Radiation Biology|
|Deposited By:||Kerstin Kopp|
|Deposited On:||12 Apr 2010 13:29|
|Last Modified:||12 Apr 2010 13:29|
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