elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Impressum | Kontakt | English
Schriftgröße: [-] Text [+]

Role of DNA Protection and Repair in Resistance of Bacillus subtilis Spores to Ultrahigh Shock Pressures Simulating Hypervelocity Impacts

Moeller, Ralf und Horneck, Gerda und Rabbow, Elke und Reitz, Günther und Meyer, Cornelia und Hornemann, Ulrich und Stöffler, Dieter (2008) Role of DNA Protection and Repair in Resistance of Bacillus subtilis Spores to Ultrahigh Shock Pressures Simulating Hypervelocity Impacts. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74 (21), Seiten 6682-6689. American Society for Microbiology. DOI: 10.1128/AEM.01091-08.

Dieses Archiv kann nicht den gesamten Text zur Verfügung stellen.

Kurzfassung

Impact-induced ejections of rocks from planetary surfaces are frequent events in the early history of the terrestrial planets and have been considered as a possible first step in the potential interplanetary transfer of microorganisms. Spores of Bacillus subtilis were used as a model system to study the effects of a simulated impact-caused ejection on rock-colonizing microorganisms using a high-explosive plane wave setup. Embedded in different types of rock material, spores were subjected to extremely high shock pressures (5 to 50 GPa) lasting for fractions of microseconds to seconds. Nearly exponential pressure response curves were obtained for spore survival and linear dependency for the induction of sporulation-defective mutants. Spores of strains defective in major small, acid-soluble spore proteins (SASP) (/-type SASP) that largely protect the spore DNA and spores of strains deficient in nonhomologous-end-joining DNA repair were significantly more sensitive to the applied shock pressure than were wild-type spores. These results indicate that DNA may be the sensitive target of spores exposed to ultrahigh shock pressures. To assess the nature of the critical physical parameter responsible for spore inactivation by ultrahigh shock pressures, the resulting peak temperature was varied by lowering the preshock temperature, changing the rock composition and porosity, or increasing the water content of the samples. Increased peak temperatures led to increased spore inactivation and reduced mutation rates. The data suggested that besides the potential mechanical stress exerted by the shock pressure, the accompanying high peak temperatures were a critical stress parameter that spores had to cope with.

Dokumentart:Zeitschriftenbeitrag
Titel:Role of DNA Protection and Repair in Resistance of Bacillus subtilis Spores to Ultrahigh Shock Pressures Simulating Hypervelocity Impacts
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Moeller, RalfNICHT SPEZIFIZIERT
Horneck, GerdaNICHT SPEZIFIZIERT
Rabbow, ElkeNICHT SPEZIFIZIERT
Reitz, GüntherNICHT SPEZIFIZIERT
Meyer, CorneliaHumboldt Universität zu Berlin, Museum für Naturkunde, Abteilung Forschung, Bereich Mineralogie, Berlin, Germany
Hornemann, UlrichFraunhofer Institute for High-Speed Dynamics, Ernst Mach Institute, Freiburg, Germany
Stöffler, DieterHumboldt Universität zu Berlin, Museum für Naturkunde, Abteilung Forschung, Bereich Mineralogie, Berlin, Germany
Datum:2008
Erschienen in:APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Referierte Publikation:Ja
In ISI Web of Science:Ja
Band:74
DOI :10.1128/AEM.01091-08
Seitenbereich:Seiten 6682-6689
Verlag:American Society for Microbiology
Status:veröffentlicht
Stichwörter:Bacillus subtilis, Hypervelocity Impacts, Ultrahigh Shock Pressures, DNA Protection, DNA Repair
HGF - Forschungsbereich:Verkehr und Weltraum (alt)
HGF - Programm:Weltraum (alt)
HGF - Programmthema:W FR - Forschung unter Weltraumbedingungen (alt)
DLR - Schwerpunkt:Weltraum
DLR - Forschungsgebiet:W FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):W - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:17 Nov 2008
Letzte Änderung:27 Apr 2009 15:27

Nur für Mitarbeiter des Archivs: Kontrollseite des Eintrags

Blättern
Suchen
Hilfe & Kontakt
Informationen
electronic library verwendet EPrints 3.3.12
Copyright © 2008-2013 Deutsches Zentrum für Luft- und Raumfahrt (DLR). Alle Rechte vorbehalten.