elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Impressum | Kontakt | English
Schriftgröße: [-] Text [+]

Characterization of different types of radiation- and pressure-induced DNA damage in Bacillus subtilis spores and their global transcriptional response during spore germination

Moeller, Ralf (2007) Characterization of different types of radiation- and pressure-induced DNA damage in Bacillus subtilis spores and their global transcriptional response during spore germination. Dissertation, Technische Universität Carolo-Wilhelmina, Braunschweig.

Dieses Archiv kann nicht den gesamten Text zur Verfügung stellen.

Kurzfassung

In general, the individual transcriptional responses of B. subtilis 168 spores to treatments by either UV-C, UV-A, X-rays, accelerated heavy ions or extreme desiccation showed certain similarities. Those corresponding responses included the induction of systems involved in DNA modification (i.e. repair, recombination, replication and packaging), in nucleotide replacement (i.e. binding, synthesis and nucleotide acid metabolism) as well as in adaptation (i.e. stress response). The similar – partly identical - responses of spores to various DNA damaging stresses point to the existence of a general “built-in”-transcriptional germination program in spores with key transcriptional events as checkpoints for ensuring DNA restoration and integrity. “Forward” spore protection mechanisms established during sporulation, such as Ca-DPA-complex, dehydration, A-DNA conformation, spore coat layers, spore core minerals, DNA binding by SASPs and the synthesis of the DNA repair systems SP lyase and NHEJ, interact with “backward” spore repair mechanisms activated or induced during germination and outgrowth, such as the reactivation of ATP- and macromoleculesbiosynthesis, (universal pathways or spore specific), active DNA repair of accumulated damage and de novo nucleotide/nucleic acid synthesis. This concerted interaction of “forward” spore protection and “backward” spore repair may constitute the major strategy of spores to maintain their high resistance towards a variety of hazardous stresses when attacked in the spore state. The identification of transcriptional and biochemical changes, elaborated in this work, has contributed substantial facets to this understanding.

Dokumentart:Hochschulschrift (Dissertation)
Titel:Characterization of different types of radiation- and pressure-induced DNA damage in Bacillus subtilis spores and their global transcriptional response during spore germination
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Moeller, RalfNICHT SPEZIFIZIERT
Datum:2007
Seitenanzahl:236
Status:nicht veröffentlicht
Stichwörter:Bacillus subtilis, Radiation, DNA damage
Institution:Technische Universität Carolo-Wilhelmina, Braunschweig
Abteilung:Fakultät für Lebenswissenschaften
HGF - Forschungsbereich:Verkehr und Weltraum (alt)
HGF - Programm:Weltraum (alt)
HGF - Programmthema:W FR - Forschung unter Weltraumbedingungen (alt)
DLR - Schwerpunkt:Weltraum
DLR - Forschungsgebiet:W FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):W - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:19 Jun 2008
Letzte Änderung:27 Apr 2009 14:35

Nur für Mitarbeiter des Archivs: Kontrollseite des Eintrags

Blättern
Suchen
Hilfe & Kontakt
Informationen
electronic library verwendet EPrints 3.3.12
Copyright © 2008-2013 Deutsches Zentrum für Luft- und Raumfahrt (DLR). Alle Rechte vorbehalten.