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The LUX-FLUORO Test as a Rapid Bioassay for Environmental Pollutants

Rettberg, Petra and Baumstark-Khan, Christa and Rabbow, Elke and Horneck, Gerda (2005) The LUX-FLUORO Test as a Rapid Bioassay for Environmental Pollutants. In: Environmental Chemistry: Green Chemistry and Pollutants in Ecosystems Springer, Berlin. pp. 645-658.

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The bioassay LUX-FLUORO test was developed for the rapid detection and quantification of environmental pollutants with genotoxic and/or cytotoxic effects. This bacterial test system uses two different reporter genes whose gene products and their reactions, respectively, can be measured easily and simultaneously by optical methods. Genotoxicity is measured by the increase of bioluminescence in genetically modified bacteria which carry a plasmid with a complete lux operon for the enzyme luciferase from the marine photobacterium P. leiognathi under the control of a DNA-damage dependent so-called SOS promoter. If the desoxyribonucleic acid (DNA) in these bacteria is damaged by a genotoxic chemical, the SOS promoter is turned on and the lux operon is expressed. The newly synthesized luciferase reacts with its substrate thereby producing bioluminescence in a damage-proportional manner. In the second part of the system, genetically modified bacteria carry the gfp gene for the green fluorescent protein from the jellyfish A. victoria downstream from a constitutively expressed promoter. These bacteria are fluorescent under normal conditions. If their cellular metabolism is disturbed by the action of cytotoxic chemicals the fluorescence decreases in a dose-proportional manner. The combined LUX-FLUORO test can be used for the biological assessment of the geno- and cytotoxicity of a wide variety of organic and inorganic chemicals including complex mixtures in different matrices.

Document Type:Book Section
Title:The LUX-FLUORO Test as a Rapid Bioassay for Environmental Pollutants
AuthorsInstitution or Email of Authors
Rettberg, PetraUNSPECIFIED
Baumstark-Khan, ChristaUNSPECIFIED
Journal or Publication Title:Environmental Chemistry: Green Chemistry and Pollutants in Ecosystems
Refereed publication:Yes
In ISI Web of Science:No
Page Range:pp. 645-658
Schwarzbauer, J.UNSPECIFIED
Publisher:Springer, Berlin
Keywords:bacterial bioassay, genotoxicity, cytotoxicity, gfp, lux, LUX-FLUORO test, SOS-LUX test, whole-cell biosensor, DNA damage
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:08 May 2007
Last Modified:27 Apr 2009 13:37

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