The LUX-FLUORO Test as a Rapid Bioassay for Environmental Pollutants
Rettberg, Petra and Baumstark-Khan, Christa and Rabbow, Elke and Horneck, Gerda (2005) The LUX-FLUORO Test as a Rapid Bioassay for Environmental Pollutants. In: Environmental Chemistry: Green Chemistry and Pollutants in Ecosystems Springer, Berlin. pp. 645-658.
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The bioassay LUX-FLUORO test was developed for the rapid detection and quantification of environmental pollutants with genotoxic and/or cytotoxic effects. This bacterial test system uses two different reporter genes whose gene products and their reactions, respectively, can be measured easily and simultaneously by optical methods. Genotoxicity is measured by the increase of bioluminescence in genetically modified bacteria which carry a plasmid with a complete lux operon for the enzyme luciferase from the marine photobacterium P. leiognathi under the control of a DNA-damage dependent so-called SOS promoter. If the desoxyribonucleic acid (DNA) in these bacteria is damaged by a genotoxic chemical, the SOS promoter is turned on and the lux operon is expressed. The newly synthesized luciferase reacts with its substrate thereby producing bioluminescence in a damage-proportional manner. In the second part of the system, genetically modified bacteria carry the gfp gene for the green fluorescent protein from the jellyfish A. victoria downstream from a constitutively expressed promoter. These bacteria are fluorescent under normal conditions. If their cellular metabolism is disturbed by the action of cytotoxic chemicals the fluorescence decreases in a dose-proportional manner. The combined LUX-FLUORO test can be used for the biological assessment of the geno- and cytotoxicity of a wide variety of organic and inorganic chemicals including complex mixtures in different matrices.
|Document Type:||Book Section|
|Title:||The LUX-FLUORO Test as a Rapid Bioassay for Environmental Pollutants|
|Journal or Publication Title:||Environmental Chemistry: Green Chemistry and Pollutants in Ecosystems|
|In ISI Web of Science:||No|
|Page Range:||pp. 645-658|
|Keywords:||bacterial bioassay, genotoxicity, cytotoxicity, gfp, lux, LUX-FLUORO test, SOS-LUX test, whole-cell biosensor, DNA damage|
|HGF - Research field:||Aeronautics, Space and Transport|
|HGF - Program:||Space|
|HGF - Program Themes:||W FR - Forschung unter Weltraumbedingungen|
|DLR - Research area:||Space|
|DLR - Program:||W FR - Forschung unter Weltraumbedingungen|
|DLR - Research theme (Project):||W - Vorhaben Strahlenbiologie (old)|
|Institutes and Institutions:||Institute of Aerospace Medicine > Radiation Biology|
|Deposited By:||Kerstin Kopp|
|Deposited On:||08 May 2007|
|Last Modified:||27 Apr 2009 13:37|
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