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Structural analysis and resistance properties of Bacillus subtilis biofilms under simulated microgravity

Fuchs, Felix M. und Holland, Gudrun und Madela, Kazimierz und Hemmersbach, Ruth und Laue, Michael und Moeller, Ralf (2017) Structural analysis and resistance properties of Bacillus subtilis biofilms under simulated microgravity. ISPS-7 & ELGRA-25 2017, 2017-10-02 - 2017-10-06, Juan les Pines, France.

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Kurzfassung

Starting with mission Apollo 16, the Gram positive bacterium Bacillus subtilis has been used in multitude of space experiments. Investigating the influence of extreme space conditions like radiation, vacuum or microgravity, experiments with model organisms like B. subtilis, which forms highly resistant endospores and biofilms, enlighten our understanding regarding survivability, resistance and potential virulence in unfavourable habitats. Biofilms are organized in a complex self-produced extracellular polymeric matrix commonly composed of polysaccharides, proteins and nucleic acids. Building a biofilm protects the individual cell against shear forces, chemicals (e.g. antibiotics or disinfectants), temperature changes and water as well as nutrient depletion (Vlamakis et al., 2013, Cairns et al., 2014). The intrinsic resistance of biofilms is challenging, not only in industry and medicine, but it can be problematic during spaceflight conditions, especially for the crew as well as for the spacecraft. In particular, long term missions with complex cooling systems, water supply and heat pipes may be vulnerable to biofilm colonisation. In our work, we used a biofilm-forming B. subtilis strain and a biofilm-matrix deficient mutant to study the impact of reduced gravity on maturated biofilms. Our major research goal is to compare biofilm formation in simulated microgravity (μg, using a fast-rotating 2D clinostat) to terrestrial gravity (1g) conditions by using different microscopic techniques. White light profilometry, scanning and transmission electron microscopy (SEM, TEM) and confocal laser scanning microscopy (CLSM) were used to analyse biofilms regarding their topology and structure, respectively. Furthermore we conducted a multitude of different survival experiments to evaluate changes and resemblances due to the impact of microgravity. First results show qualitative architectural differences between simulated microgravity and 1g in cross-sections, but no significant qualitative variations in biofilm surface topography. Biofilms grown under simulated microgravity seem to exhibit similar extreme resistances to environmental changes, compared to 1g-controls, when exposed to space-like conditions.

elib-URL des Eintrags:https://elib.dlr.de/117712/
Dokumentart:Konferenzbeitrag (Vortrag)
Titel:Structural analysis and resistance properties of Bacillus subtilis biofilms under simulated microgravity
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Fuchs, Felix M.German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Cologne (Köln), Germany; Felix.fuchs (at) dlr.dehttps://orcid.org/0000-0001-5669-5655NICHT SPEZIFIZIERT
Holland, GudrunRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Madela, KazimierzRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Hemmersbach, Ruth3German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Gravitational Biology, Cologne (Köln), Germanyhttps://orcid.org/0000-0001-5308-6715NICHT SPEZIFIZIERT
Laue, MichaelRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Moeller, RalfGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Cologne (Köln), Germany; E-mail: ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676NICHT SPEZIFIZIERT
Datum:2017
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Nein
Status:veröffentlicht
Stichwörter:biofilm, B. subtilis, spores, microgravity, clinostat, SEM, CLSM, profilometry
Veranstaltungstitel:ISPS-7 & ELGRA-25 2017
Veranstaltungsort:Juan les Pines, France
Veranstaltungsart:internationale Konferenz
Veranstaltungsbeginn:2 Oktober 2017
Veranstaltungsende:6 Oktober 2017
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Artificial Gravity (alt), R - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Gravitationsbiologie
Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:11 Jan 2018 13:40
Letzte Änderung:24 Apr 2024 20:22

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