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Biofilm formation and sporulation of Bacillus subtilis under simulated microgravity

Fuchs, Felix M. und Holland, Gudrun und Madela, Kazimierz und Falcón García, Carolina und Hemmersbach, Ruth und Laue, Michael und Lieleg, Oliver und Moeller, Ralf (2017) Biofilm formation and sporulation of Bacillus subtilis under simulated microgravity. 19th International Conference on Bacilli & Gram-Positive Bacteria, 2017-06-11 - 2017-06-15, Berlin, Germany.

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Kurzfassung

Since humans are travelling into the outer atmospheres of our planet and beyond, a new environmental habitat was established. Knowing that life - possibly since its first appearance on Earth is adapted to terrestrial gravity, the impact of microgravity on prokaryotes and humans remains mostly unclear. Investigating the influence of extreme conditions like those in space, Bacillus subtilis was chosen as model organism. Non-domesticated strains, such as NCIB 3610 have the ability to form biofilms as well as highly resistant endospores. Since it is known that planktonic life is the exception, biofilms are considered as predominant way of living (Moons et al., 2009). Building a biofilm protects the individual cell against shear forces, chemicals (e.g. antibiotics or disinfectants), temperature changes and water as well as nutrient depletion (Vlamakis et al., 2013, Cairns et al., 2014). The intrinsic resistance of biofilms is a problem, not only in industry and medicine, but it can be problematic during spaceflight conditions, especially for the crew as well as for the spacecraft. In particular, long term missions with complex cooling systems, water supply and heat pipes may be vulnerable to biofilm colonisation. In our work, we used a rotating 2D-clinostat to determine the effect of simulated microgravity (sim.-μg) and terrestrial gravity (1g) on B. subtilis biofilms and spores by using different microscopic techniques. White light profilometry, scanning and transmission electron microscopy (SEM, TEM) and confocal laser scanning microscopy (CLSM) were used to analyse biofilms regarding their topology and inner structure, respectively. First results show qualitative architectural differences between simulated microgravity and 1g in cross-sections, but no significant qualitative variations in biofilm surface topography. In order to test the resistance of spores grown under simulated gravity, germination assays, as well as survival assays were used. First results revealed that spores grown under the influence of sim.-μg had an increased spontaneous germination rate compared to spores grown at 1g.

elib-URL des Eintrags:https://elib.dlr.de/117704/
Dokumentart:Konferenzbeitrag (Poster)
Titel:Biofilm formation and sporulation of Bacillus subtilis under simulated microgravity
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Fuchs, Felix M.German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology, Space Microbiology Research Group, Cologne (Köln), Germany; Felix.fuchs (at) dlr.dehttps://orcid.org/0000-0001-5669-5655NICHT SPEZIFIZIERT
Holland, GudrunRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Madela, KazimierzRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Falcón García, CarolinaTechnische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715NICHT SPEZIFIZIERT
Laue, MichaelRobert Koch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Lieleg, OliverTechnische Universität München (TUM), Department of Mechanical Engineering and Munich School of Bioengineering, Garching, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Moeller, RalfGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology, Space Microbiology Research Group, Cologne (Köln), Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676NICHT SPEZIFIZIERT
Datum:2017
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Nein
Status:veröffentlicht
Stichwörter:Bacillus subtilis, microgravity
Veranstaltungstitel:19th International Conference on Bacilli & Gram-Positive Bacteria
Veranstaltungsort:Berlin, Germany
Veranstaltungsart:internationale Konferenz
Veranstaltungsbeginn:11 Juni 2017
Veranstaltungsende:15 Juni 2017
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Artificial Gravity (alt), R - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Gravitationsbiologie
Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:11 Jan 2018 13:39
Letzte Änderung:24 Apr 2024 20:22

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